Homo sapiens Protein: DDX58
InnateDB Protein IDBP-55856.6
Last Modified 2014-10-13 [Report errors or provide feedback]
Gene Symbol DDX58
Protein Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Synonyms RIG-I; RIGI; RLR-1;
Species Homo sapiens
Ensembl Protein ENSP00000369213
InnateDB Gene IDBG-55854 (DDX58)
Protein Structure
UniProt Annotation
Function Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5'- triphosphorylated ssRNA and dsRNA and short dsRNA (<1 kb in length). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK- related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae: Human respiratory syncytial virus and measles virus (MeV), Rhabdoviridae: vesicular stomatitis virus (VSV), Orthomyxoviridae: influenza A and B virus, Flaviviridae: Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV). It also detects rotavirus and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration. {ECO:0000269PubMed:15208624, ECO:0000269PubMed:15708988, ECO:0000269PubMed:16125763, ECO:0000269PubMed:16127453, ECO:0000269PubMed:16153868, ECO:0000269PubMed:17190814, ECO:0000269PubMed:18636086, ECO:0000269PubMed:19122199, ECO:0000269PubMed:19211564, ECO:0000269PubMed:19576794, ECO:0000269PubMed:19609254, ECO:0000269PubMed:19631370, ECO:0000269PubMed:21742966}.
Subcellular Localization Cytoplasm. Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell junction, tight junction. Note=Colocalized with TRIM25 at cytoplasmic perinuclear bodies. Associated with the actin cytoskeleton at membrane ruffles.
Disease Associations
Tissue Specificity Present in vascular smooth cells (at protein level). {ECO:0000269PubMed:15219805}.
Number of Interactions This gene and/or its encoded proteins are associated with 119 experimentally validated interaction(s) in this database.
They are also associated with 19 interaction(s) predicted by orthology.
Experimentally validated
Total 119 [view]
Protein-Protein 108 [view]
Protein-DNA 7 [view]
Protein-RNA 2 [view]
RNA-RNA 1 [view]
DNA-RNA 1 [view]
Predicted by orthology
Total 19 [view]
Gene Ontology

Molecular Function
Accession GO Term
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003690 double-stranded DNA binding
GO:0003725 double-stranded RNA binding
GO:0003727 single-stranded RNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016817 hydrolase activity, acting on acid anhydrides
GO:0042802 identical protein binding
Biological Process
GO:0002230 positive regulation of defense response to virus by host
GO:0008152 metabolic process
GO:0009597 detection of virus
GO:0009615 response to virus
GO:0016032 viral process
GO:0030334 regulation of cell migration
GO:0032480 negative regulation of type I interferon production
GO:0032727 positive regulation of interferon-alpha production
GO:0032728 positive regulation of interferon-beta production
GO:0034344 regulation of type III interferon production
GO:0039528 cytoplasmic pattern recognition receptor signaling pathway in response to virus
GO:0039529 RIG-I signaling pathway
GO:0042993 positive regulation of transcription factor import into nucleus
GO:0043330 response to exogenous dsRNA
GO:0045087 innate immune response (InnateDB)
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0051091 positive regulation of sequence-specific DNA binding transcription factor activity
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005923 tight junction
GO:0015629 actin cytoskeleton
GO:0032587 ruffle membrane
Protein Structure and Domains
InterPro IPR001650 Helicase, C-terminal
IPR006935 Helicase/UvrB domain
IPR011029 Death-like domain
IPR011545 DEAD/DEAH box helicase domain
IPR014001 Helicase, superfamily 1/2, ATP-binding domain
IPR021673 C-terminal domain of RIG-I
IPR027417 P-loop containing nucleoside triphosphate hydrolase
PFAM PF00271
Post-translational Modifications
SwissProt O95786
PhosphoSite PhosphoSite-O95786
UniProt Splice Variant
Entrez Gene 23586
UniGene Hs.609798
RefSeq NP_055129
OMIM 609631
HPRD 13131
EMBL AF038963 AL137608 AL161783 AL353671 BC132786 BC136610 BX647917 CH471071
GenPept AAD19826 AAI32787 AAI36611 CAB70840 CAH71251 CAH72600 CAI46068 EAW58548