InnateDB: Systems Biology of the Innate Immune Response

Bos taurus Protein: NME2

Summary

InnateDB Protein

IDBP-690505.2

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

NME2

Protein Name

Nucleoside diphosphate kinase B

Synonyms

Species

Bos taurus

Ensembl Protein

ENSBTAP00000041066

InnateDB Gene

IDBG-638435 (NME2)

Protein Structure

UniProt Annotation

Function

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Negatively regulates Rho activity by interacting with AKAP13/LBC (By similarity). Exhibits histidine protein kinase activity. {ECO:0000250, ECO:0000269PubMed:12486123}.

Subcellular Localization

Cytoplasm. Cell membrane {ECO:0000250}. Cell projection, lamellipodium {ECO:0000250}. Cell projection, ruffle {ECO:0000250}. Note=Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates. {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 2 experimentally validated interaction(s) in this database.
They are also associated with 58 interaction(s) predicted by orthology.

Experimentally validated
Total	2 [view]
Protein-Protein	2 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	58 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000166	nucleotide binding
GO:0004550	nucleoside diphosphate kinase activity
GO:0004673	protein histidine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016301	kinase activity
GO:0016740	transferase activity
GO:0046872	metal ion binding

Biological Process

GO:0006165	nucleoside diphosphate phosphorylation
GO:0006183	GTP biosynthetic process
GO:0006228	UTP biosynthetic process
GO:0006241	CTP biosynthetic process
GO:0007229	integrin-mediated signaling pathway
GO:0009117	nucleotide metabolic process
GO:0009142	nucleoside triphosphate biosynthetic process
GO:0016310	phosphorylation
GO:0018106	peptidyl-histidine phosphorylation
GO:0043066	negative regulation of apoptotic process
GO:0045618	positive regulation of keratinocyte differentiation
GO:0045682	regulation of epidermis development
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0050679	positive regulation of epithelial cell proliferation

Cellular Component

GO:0001726	ruffle
GO:0005634	nucleus
GO:0005737	cytoplasm
GO:0005886	plasma membrane
GO:0016020	membrane
GO:0030027	lamellipodium
GO:0042995	cell projection
GO:0070062	extracellular vesicular exosome
GO:0071944	cell periphery

Protein Structure and Domains

PDB ID

InterPro

IPR001564 Nucleoside diphosphate kinase

PFAM

PF00334

PRINTS

PR01243

PIRSF

SMART

SM00562

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q3T0Q4

PhosphoSite

PhosphoSite-

TrEMBL

F1MPL4

UniProt Splice Variant

Entrez Gene

615447

UniGene

RefSeq

NP_001069844

HUGO

OMIM

CCDS

HPRD

IMGT

EMBL

BC102300 DAAA02049024

GenPept

AAI02301