InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RUVBL1

Summary

InnateDB Protein

IDBP-55110.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RUVBL1

Protein Name

RuvB-like 1 (E. coli)

Synonyms

ECP54; INO80H; NMP238; PONTIN; Pontin52; RVB1; TIH1; TIP49; TIP49A;

Species

Homo sapiens

Ensembl Protein

ENSP00000318297

InnateDB Gene

IDBG-55108 (RUVBL1)

Protein Structure

UniProt Annotation

Function

Possesses single-stranded DNA-stimulated ATPase and ATP- dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity.Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome.Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.Plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. Essential for cell proliferation.May be able to bind plasminogen at cell surface and enhance plasminogen activation.

Subcellular Localization

Nucleus matrix. Nucleus, nucleoplasm. Cytoplasm. Membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells.

Disease Associations

Tissue Specificity

Ubiquitously expressed with high expression in heart, skeletal muscle and testis.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 207 experimentally validated interaction(s) in this database.
They are also associated with 6 interaction(s) predicted by orthology.

Experimentally validated
Total	208 [view]
Protein-Protein	205 [view]
Protein-DNA	2 [view]
Protein-RNA	0
DNA-DNA	1 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	6 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003678	DNA helicase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0009378	four-way junction helicase activity
GO:0043141	ATP-dependent 5'-3' DNA helicase activity

Biological Process

GO:0006281	DNA repair
GO:0006310	DNA recombination
GO:0006325	chromatin organization
GO:0006334	nucleosome assembly
GO:0006351	transcription, DNA-templated
GO:0006357	regulation of transcription from RNA polymerase II promoter
GO:0007067	mitotic nuclear division
GO:0007283	spermatogenesis
GO:0032508	DNA duplex unwinding
GO:0034080	centromere-specific nucleosome assembly
GO:0040008	regulation of growth
GO:0043967	histone H4 acetylation
GO:0043968	histone H2A acetylation

Cellular Component

GO:0000812	Swr1 complex
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0005737	cytoplasm
GO:0005794	Golgi apparatus
GO:0005815	microtubule organizing center
GO:0016020	membrane
GO:0016363	nuclear matrix
GO:0030529	ribonucleoprotein complex
GO:0031011	Ino80 complex
GO:0035267	NuA4 histone acetyltransferase complex
GO:0043231	intracellular membrane-bounded organelle
GO:0043234	protein complex
GO:0070062	extracellular vesicular exosome
GO:0071339	MLL1 complex

Protein Structure and Domains

PDB ID

InterPro

IPR003593 AAA+ ATPase domain
IPR003959 ATPase, AAA-type, core
IPR008824 DNA helicase, Holliday junction RuvB type, N-terminal
IPR010339 TIP49, C-terminal
IPR012340 Nucleic acid-binding, OB-fold
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF00004
PF07724
PF13304
PF05496
PF06068

PRINTS

PIRSF

SMART

SM00382

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

Q9Y265