Version 5.4
Interaction: HIF1A :: SP1 XML
Summary
InnateDB Group ID 169702
Short name HIF1A :: SP1
Full name HIF1A interacts with SP1
Interaction type physical association
Participants
Participant 1
Molecule: IDBG-36616
Molecule type: protein
Species: Homo sapiens
HUGO: SP1
Full name: Sp1 transcription factor
Direction: input
Biological role: unspecified role
Participant 2
Molecule: IDBG-8510
Molecule type: protein
Species: Homo sapiens
HUGO: HIF1A
Full name: hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Direction: input
Biological role: unspecified role
Evidence Reference 1
InnateDB ID IDB-82821.3
Last Modified 2009-09-10
Review status Unreviewed by InnateDB [Report errors or provide feedback]
Source database MINT
Source database ID MINT-3378397
PMID 17024177
Interaction detection method anti bait coimmunoprecipitation
Host system invitro
Host organism
Cell status
Cell line
Cell type
Tissue type
Comment
Participants
Participant Identification Method Experimental Role Participant Accession
1) SP1 western blot bait P08047
2) HIF1A nucleotide sequence identification prey Q16665
Evidence Reference 2
InnateDB ID IDB-82822.3
Last Modified 2009-09-10
Review status Unreviewed by InnateDB [Report errors or provide feedback]
Source database MINT
Source database ID MINT-3378435
PMID 17024177
Interaction detection method competition binding
Host system invitro
Host organism
Cell status
Cell line
Cell type
Tissue type
Comment
Participants
Participant Identification Method Experimental Role Participant Accession
1) HIF1A nucleotide sequence identification prey Q16665
2) SP1 western blot bait P08047
Evidence Reference 3
InnateDB ID IDB-82823.3
Last Modified 2009-09-10
Review status Unreviewed by InnateDB [Report errors or provide feedback]
Source database MINT
Source database ID MINT-3378454
PMID 17024177
Interaction detection method anti bait coimmunoprecipitation
Host system invitro
Host organism
Cell status
Cell line
Cell type
Tissue type
Comment
Participants
Participant Identification Method Experimental Role Participant Accession
1) SP1 western blot bait P08047
2) HIF1A nucleotide sequence identification prey Q16665
Evidence Reference 4
InnateDB ID IDB-120549.2
Last Modified 2010-03-07
Review status reviewed [Report errors or provide feedback]
Source database INNATEDB
Source database ID IDB-120549
PMID 12228247
Interaction detection method coimmunoprecipitation
Host system invitro
Host organism Homo sapiens
Cell status cell line
Cell line HeLa
Cell type epithelial cell
Tissue type cervical cancer cell line
Comment HIF1A interacts with SP1 weakly when cells were treated with hypoxia, but stronger when cells were treated with hypoxia and TGF-beta1.
Participants
Participant Identification Method Experimental Role Participant Accession
1) HIF1A western blot bait
2) SP1 western blot prey
Evidence Reference 5
InnateDB ID IDB-293008.1
Last Modified 2012-02-14
Review status Unreviewed by InnateDB [Report errors or provide feedback]
Source database BIOGRID
Source database ID BIOGRID-592290
PMID 17024177
Interaction detection method affinity chromatography technology
Host system invitro
Host organism
Cell status
Cell line
Cell type
Tissue type
Comment
Participants
Participant Identification Method Experimental Role Participant Accession
1) SP1 predetermined participant bait 6667
2) HIF1A predetermined participant prey 3091
Evidence Reference 6
InnateDB ID IDB-1068833.1
Last Modified 2013-07-09
Review status Unreviewed by InnateDB [Report errors or provide feedback]
Source database BIOGRID
Source database ID BIOGRID-854583
PMID 12228247
Interaction detection method affinity chromatography technology
Host system invitro
Host organism
Cell status
Cell line
Cell type
Tissue type
Comment
Participants
Participant Identification Method Experimental Role Participant Accession
1) HIF1A predetermined participant bait 3091
2) SP1 predetermined participant prey 6667
Evidence Reference 7
InnateDB ID IDB-1081232.1
Last Modified 2013-07-09
Review status Unreviewed by InnateDB [Report errors or provide feedback]
Source database BIOGRID
Source database ID BIOGRID-854581
PMID 12228247
Interaction detection method affinity chromatography technology
Host system invitro
Host organism
Cell status
Cell line
Cell type
Tissue type
Comment
Participants
Participant Identification Method Experimental Role Participant Accession
1) SP1 predetermined participant bait 6667
2) HIF1A predetermined participant prey 3091
Evidence Reference 8
InnateDB ID IDB-1653346.1
Last Modified 2014-11-29
Review status Unreviewed by InnateDB [Report errors or provide feedback]
Source database INTACT
Source database ID EBI-7015964
PMID 17024177
Interaction detection method anti bait coimmunoprecipitation
Host system invitro
Host organism
Cell status
Cell line
Cell type
Tissue type
Comment
Participants
Participant Identification Method Experimental Role Participant Accession
1) SP1 western blot bait P08047
2) HIF1A nucleotide sequence identification prey Q16665
Evidence Reference 9
InnateDB ID IDB-1653348.1
Last Modified 2014-11-29
Review status Unreviewed by InnateDB [Report errors or provide feedback]
Source database INTACT
Source database ID EBI-7016025
PMID 17024177
Interaction detection method anti bait coimmunoprecipitation
Host system invitro
Host organism
Cell status
Cell line
Cell type
Tissue type
Comment
Participants
Participant Identification Method Experimental Role Participant Accession
1) SP1 western blot bait P08047
2) HIF1A nucleotide sequence identification prey Q16665
Evidence Reference 10
InnateDB ID IDB-1653349.1
Last Modified 2014-11-29
Review status Unreviewed by InnateDB [Report errors or provide feedback]
Source database INTACT
Source database ID EBI-7016111
PMID 17024177
Interaction detection method competition binding
Host system invitro
Host organism
Cell status
Cell line
Cell type
Tissue type
Comment
Participants
Participant Identification Method Experimental Role Participant Accession
1) HIF1A nucleotide sequence identification prey Q16665
2) SP1 western blot bait P08047
Evidence Reference 11
InnateDB ID IDB-2063538.1
Last Modified 2018-01-20
Review status reviewed [Report errors or provide feedback]
Source database INNATEDB
Source database ID IDB-2063538
PMID 15780936
Interaction detection method anti bait coimmunoprecipitation
Host system invitro
Host organism Homo sapiens
Cell status cell line
Cell line HCT116
Cell type
Tissue type
Comment Figure 5B.
Participants
Participant Identification Method Experimental Role Participant Accession
1) SP1 identification by antibody bait
2) HIF1A western blot prey
Evidence Reference 12
InnateDB ID IDB-2063538.1
Last Modified 2018-01-20
Review status reviewed [Report errors or provide feedback]
Source database INNATEDB ALLERGY
Source database ID AAP-2063538
PMID 15780936
Interaction detection method anti bait coimmunoprecipitation
Host system invitro
Host organism Homo sapiens
Cell status cell line
Cell line HCT116
Cell type
Tissue type
Comment Figure 5B.
Participants
Participant Identification Method Experimental Role Participant Accession
1) SP1 identification by antibody bait
2) HIF1A western blot prey
Evidence Reference 13
InnateDB ID IDB-2063539.1
Last Modified 2018-01-20
Review status reviewed [Report errors or provide feedback]
Source database INNATEDB
Source database ID IDB-2063539
PMID 15780936
Interaction detection method anti bait coimmunoprecipitation
Host system invitro
Host organism Homo sapiens
Cell status cell line
Cell line HCT116
Cell type
Tissue type
Comment Figure 5C. 35S methionine-labelled HIF1A co-immunnoprecipitated with anti-SP1.
Participants
Participant Identification Method Experimental Role Participant Accession
1) SP1 identification by antibody bait
2) HIF1A autoradiography prey
Evidence Reference 14
InnateDB ID IDB-2063539.1
Last Modified 2018-01-20
Review status reviewed [Report errors or provide feedback]
Source database INNATEDB ALLERGY
Source database ID AAP-2063539
PMID 15780936
Interaction detection method anti bait coimmunoprecipitation
Host system invitro
Host organism Homo sapiens
Cell status cell line
Cell line HCT116
Cell type
Tissue type
Comment Figure 5C. 35S methionine-labelled HIF1A co-immunnoprecipitated with anti-SP1.
Participants
Participant Identification Method Experimental Role Participant Accession
1) SP1 identification by antibody bait
2) HIF1A autoradiography prey

If you use InnateDB for your research, please cite the following publication:

Breuer et al., InnateDB: systems biology of innate immunity and beyond - recent updates and continuing curation. Nucl. Acids Res. (2013) 41 (D1) 

InnateDB is being developed jointly by the Brinkman Laboratory (Simon Fraser University, British Columbia, Canada), the Hancock Laboratory (University of British Columbia, Vancouver, British Columbia) and the Lynn EMBL Australia Group (South Australian Health & Medical Research Institute and Flinders University, Adelaide, Australia).

Funding is currently provided by Allergen and EMBL Australia. Previous funding has been provided by Genome Canada, the Foundation for the National Institutes of Health through the Grand Challenges in Global Health initiative and by Teagasc. InnateDB curated interactions are licensed under the Design Science License. All other data is licensed under the terms of the originating database. Contact: innatedb-mail@sfu.ca