Interaction: FBXW7 :: JUN XML
Summary
InnateDB Group ID 134623
Short name FBXW7 :: JUN
Full name FBXW7 interacts with JUN
Interaction type physical association
Participants
Participant 1
Molecule: IDBG-41233
Molecule type: protein
Species: Homo sapiens
HUGO: FBXW7
Full name: F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase
Direction: input
Biological role: unspecified role
Participant 2
Molecule: IDBG-99221
Molecule type: protein
Species: Homo sapiens
HUGO: JUN
Full name: jun proto-oncogene
Direction: input
Biological role: unspecified role
Evidence Reference 1
InnateDB ID IDB-2063521.1
Last Modified 2017-12-23
Review status reviewed [Report errors or provide feedback]
Source database INNATEDB ALLERGY
Source database ID AAP-2063521
PMID 16023596
Interaction detection method pull down
Host system invitro
Host organism
Cell status
Cell line
Cell type
Tissue type
Comment Figure 1C. Biotinylated JUN 239p 243p was bound to streptavidin agarose and then incubated with 35S-labelled FBXW7 for 1 hour at 4C. Bound proteins were detected by autoradiography.
Participants
Participant Identification Method Experimental Role Participant Accession
1) FBXW7 autoradiography prey
2) JUN predetermined participant bait
Evidence Reference 2
InnateDB ID IDB-2063522.1
Last Modified 2018-01-13
Review status reviewed [Report errors or provide feedback]
Source database INNATEDB ALLERGY
Source database ID AAP-2063522
PMID 16023596
Interaction detection method anti tag coimmunoprecipitation
Host system invitro
Host organism Homo sapiens
Cell status cell line
Cell line HeLa
Cell type
Tissue type
Comment Figure 1F. Cells were transfected with HA-FBXW7 and Myc tagged JUN.
Participants
Participant Identification Method Experimental Role Participant Accession
1) FBXW7 anti tag western blot prey
2) JUN anti tag western blot bait
Evidence Reference 3
InnateDB ID IDB-2063523.1
Last Modified 2018-01-13
Review status reviewed [Report errors or provide feedback]
Source database INNATEDB ALLERGY
Source database ID AAP-2063523
PMID 16023596
Interaction detection method anti tag coimmunoprecipitation
Host system invitro
Host organism Homo sapiens
Cell status cell line
Cell line HeLa
Cell type
Tissue type
Comment Figure 1G. Cells were transfected with HA-FBXW7 and Flag-tagged JUN.
Participants
Participant Identification Method Experimental Role Participant Accession
1) FBXW7 anti tag western blot prey
2) JUN anti tag western blot bait
Evidence Reference 4
InnateDB ID IDB-2063524.1
Last Modified 2018-01-13
Review status reviewed [Report errors or provide feedback]
Source database INNATEDB ALLERGY
Source database ID AAP-2063524
PMID 16023596
Interaction detection method anti tag coimmunoprecipitation
Host system invitro
Host organism Homo sapiens
Cell status cell line
Cell line 293T
Cell type
Tissue type
Comment Figure 1H. Cells were transfected with HA-FBXW7.
Participants
Participant Identification Method Experimental Role Participant Accession
1) FBXW7 anti tag western blot bait
2) JUN western blot prey