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InnateDB Group ID
|
134623
|
|
Short name
|
FBXW7 :: JUN
|
|
Full name
|
FBXW7 interacts with JUN
|
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Interaction type
|
physical association
|
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Participant 1
|
|
Molecule:
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IDBG-41233
|
|
Molecule type:
|
protein
|
|
Species:
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Homo sapiens
|
|
HUGO:
|
FBXW7
|
|
Full name:
|
F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase
|
|
Direction:
|
input
|
|
Biological role:
|
unspecified role
|
|
Participant 2
|
|
Molecule:
|
IDBG-99221
|
|
Molecule type:
|
protein
|
|
Species:
|
Homo sapiens
|
|
HUGO:
|
JUN
|
|
Full name:
|
jun proto-oncogene
|
|
Direction:
|
input
|
|
Biological role:
|
unspecified role
|
|
InnateDB ID
|
IDB-2063521.1
|
|
Last Modified
|
2017-12-23
|
|
Review status
|
reviewed
[Report errors or provide feedback]
|
|
Source database
|
INNATEDB ALLERGY
|
|
Source database ID
|
AAP-2063521
|
|
PMID
|
16023596
|
|
Interaction detection method
|
pull down |
|
Host system
|
invitro |
|
Host organism
|
|
|
Cell status
|
|
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Cell line
|
|
|
Cell type
|
|
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Tissue type
|
|
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Comment
|
Figure 1C. Biotinylated JUN 239p 243p was bound to streptavidin agarose and then incubated with 35S-labelled FBXW7 for 1 hour at 4C. Bound proteins were detected by autoradiography. |
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Participants
|
|
Participant Identification Method |
Experimental Role |
Participant Accession |
| 1) FBXW7 |
autoradiography |
prey |
|
| 2) JUN |
predetermined participant |
bait |
|
|
|
InnateDB ID
|
IDB-2063522.1
|
|
Last Modified
|
2018-01-13
|
|
Review status
|
reviewed
[Report errors or provide feedback]
|
|
Source database
|
INNATEDB ALLERGY
|
|
Source database ID
|
AAP-2063522
|
|
PMID
|
16023596
|
|
Interaction detection method
|
anti tag coimmunoprecipitation |
|
Host system
|
invitro |
|
Host organism
|
Homo sapiens |
|
Cell status
|
cell line |
|
Cell line
|
HeLa |
|
Cell type
|
|
|
Tissue type
|
|
|
Comment
|
Figure 1F. Cells were transfected with HA-FBXW7 and Myc tagged JUN. |
|
Participants
|
|
Participant Identification Method |
Experimental Role |
Participant Accession |
| 1) FBXW7 |
anti tag western blot |
prey |
|
| 2) JUN |
anti tag western blot |
bait |
|
|
|
InnateDB ID
|
IDB-2063523.1
|
|
Last Modified
|
2018-01-13
|
|
Review status
|
reviewed
[Report errors or provide feedback]
|
|
Source database
|
INNATEDB ALLERGY
|
|
Source database ID
|
AAP-2063523
|
|
PMID
|
16023596
|
|
Interaction detection method
|
anti tag coimmunoprecipitation |
|
Host system
|
invitro |
|
Host organism
|
Homo sapiens |
|
Cell status
|
cell line |
|
Cell line
|
HeLa |
|
Cell type
|
|
|
Tissue type
|
|
|
Comment
|
Figure 1G. Cells were transfected with HA-FBXW7 and Flag-tagged JUN. |
|
Participants
|
|
Participant Identification Method |
Experimental Role |
Participant Accession |
| 1) FBXW7 |
anti tag western blot |
prey |
|
| 2) JUN |
anti tag western blot |
bait |
|
|
|
InnateDB ID
|
IDB-2063524.1
|
|
Last Modified
|
2018-01-13
|
|
Review status
|
reviewed
[Report errors or provide feedback]
|
|
Source database
|
INNATEDB ALLERGY
|
|
Source database ID
|
AAP-2063524
|
|
PMID
|
16023596
|
|
Interaction detection method
|
anti tag coimmunoprecipitation |
|
Host system
|
invitro |
|
Host organism
|
Homo sapiens |
|
Cell status
|
cell line |
|
Cell line
|
293T |
|
Cell type
|
|
|
Tissue type
|
|
|
Comment
|
Figure 1H. Cells were transfected with HA-FBXW7. |
|
Participants
|
|
Participant Identification Method |
Experimental Role |
Participant Accession |
| 1) FBXW7 |
anti tag western blot |
bait |
|
| 2) JUN |
western blot |
prey |
|
|
|
|
|
Version 5.4